TRIS HCl, CAS: 1185-53-1

Tris(hydroxymethyl)aminomethane hydrochloride

is an organic compound with the molecular formula C4H11NO3 and a molecular weight of 157.6.

TRIS HCl is an important biological buffer, and can also be used as an intermediate in organic synthesis. Additionally, it can be utilized for protein crystallization under different pH conditions, the formation of intermediate filaments of nuclear envelope proteins in nematodes, as well as the preparation of surfactants, vulcanization accelerators, and some pharmaceutical intermediates.

The invention of CN109468311B discloses a method for extracting plasmid DNA, in which TRIS HCl solution is widely used as a solvent for nucleic acids and proteins, and also constitutes a part of the buffer system for cell lysis. The specific technical solution of this invention is as follows:

The bacterial lysis solution for extracting plasmid DNA consists of lysis buffer, lysozyme, and RNase A. The composition of the lysis buffer is specifically: for every 100mL of lysis buffer, it contains 1.5 to 10g of sucrose, 0.7 to 2.2g of disodium ethylenediaminetetraacetate, 0.3 to 0.9g of TRIS HCl, 2.7 to 5.4g of NH4Cl, 0.1 to 2.0mL of 0.5% Triton X-100, 0.2 to 0.6g of CaCl2, 0.5 to 8.0g of guanidine hydrochloride, 0.5 to 3.0g of caprylic acid thiocyanate, and the remainder being water. The pH of the bacterial lysis solution is in the range of 5.0 to 8.0.

The unique bacterial lysate disclosed in the present invention improves the lysis efficiency, reduces contamination caused by proteins and RNA, and also enhances the binding efficiency of plasmid DNA to the centrifugal adsorption column.

References:

CN109468311B Bacterial lysate for extracting plasmid DNA, reagent kit, and method for extracting plasmid DNA