Skip to content 
BioBuffer
MES Monohydrate
CAS: 145224-94-8 | Code: DL023002
2-(N-Morpholino)
ethanesulfonic acid,monohydrate
CAS: 145224-94-8
A zwitterionic biological buffer is often used as a buffering agent in biological and biochemical research.
- Cell Culture Media
- TBE Buffer
| ITEMS | SPECIFICATION |
|---|---|
| IUPAC Name | 2-(N-Morpholino)ethanesulfonic acid, monohydrate |
| Appearance : | White crystalline powder |
| CAS NO: | 145224-94-8 |
| Purity: | >99.5% |
| Chemical Formula: | C6H13NO4S.H2O |
| Mol. Weight : | 213.25 g/mol |
| Useful pH Range: | 5.5~6.7 |
| pKa (25 °C): | 6.1 |
| Packages: | 500g/1Kg/25Kg/Custom Packaging |
MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions.
MES free acid or MES free acid monohydrate can be mixed with MES sodium salt to acquire desired pH. Alternatively, MES free acid or MES free acid monohydrate can be titrated with sodium hydroxide to attain desired pH.
