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Are you unsure how to select these three TMB derivatives?
TMB (Free Base, CAS 54827-17-7)
TMB Dihydrochloride (CAS 207738-08-7)
TMB Sulfate (CAS 54827-18-8)
Here’s TMB Derivatives Selection and Application Guide from DALO CHEM for your reference.
1. Chemical Properties and Solubility
TMB (Free Base, CAS 54827-17-7)
Insoluble in water; requires organic solvents (e.g., DMSO, ethanol) for dissolution.
Lower stability; prone to oxidation and discoloration during long-term storage.
Higher volatility; handle in a fume hood.
TMB Dihydrochloride (CAS 207738-08-7)
Highly water-soluble, ideal for direct preparation of aqueous solutions (e.g., ELISA buffers).
Better stability with a longer shelf life.
Broad pH compatibility for chromogenic reactions (commonly used at pH 4-5).
TMB Sulfate (CAS 54827-18-8)
Moderate water solubility but offers enhanced stability once dissolved.
Higher sensitivity in strongly acidic conditions (suitable for specific pH requirements).
Cl⁻-free, preferred for chloride-sensitive systems (e.g., electrochemical assays).
2. Applications
TMB (Free Base)
Suitable for organic solvent-based systems (e.g., chemical synthesis or non-aqueous detection).
Requires manual optimization of reaction conditions (e.g., adding H₂O₂ and acid).
TMB Dihydrochloride
Widely used as an ELISA substrate; reacts with HRP (horseradish peroxidase) to produce a blue color.
Common in commercial kits due to ease of use.
TMB Sulfate
Ideal for chloride-sensitive experiments (e.g., systems using Ag/AgCl electrodes).
Provides stable chromogenic signals in prolonged assays.
3. Selection Guidelines
Choose TMB Dihydrochloride if:
Performing routine ELISA, immunohistochemistry, or enzyme-linked assays.
Prioritizing fast dissolution and simplicity.
Working with cost-sensitive experiments (typically more affordable).
Choose TMB Sulfate if:
The assay is sensitive to chloride ions (e.g., electrochemical setups).
Optimizing sensitivity in strong acidic conditions.
Requiring long-term storage of chromogenic solutions (superior stability).
Choose TMB Free Base if:
Conducting reactions in organic solvents.
Customizing reaction conditions (e.g., adjusting acidity and oxidizers).
4. Safety and Storage
Toxicity: All three derivatives are potentially carcinogenic; avoid direct contact.
Storage:
Salt forms (dihydrochloride/sulfate): Store in dark, dry conditions (2-8°C recommended).
Free base: Seal tightly to prevent oxidation (nitrogen-purged storage advised).
Summary
General assays (e.g., ELISA): Opt for TMB Dihydrochloride (cost-effective and user-friendly).
Specialized requirements: Select TMB Sulfate based on pH, chloride sensitivity, or stability needs.
Non-aqueous systems: Use TMB Free Base with proper safety precautions.
We advise you to choose based on specific experimental needs (solubility, pH, stability, cost). For initial trials, TMB Dihydrochloride is recommended as a universal option.
DALO CHEM provides all this three TMB products. Welcome to inquiry!
