Biobuffer
HEPES
CAS: 7365-45-9 | Code: DL023024
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
HEPES is a widely used zwitterionic biological buffer has excellent buffering capacity and minimal interference with biological processes, making it an essential reagent in various biochemical and molecular biology applications.
- Cell Culture Media
- Chromatography
- Diagnostic Tests
- Electrophoresis
- Pharmaceutical
| ITEMS | SPECIFICATION |
|---|---|
| IUPAC Name | 4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid |
| Appearance : | White crystalline powder |
| Code: | DL023033 |
| CAS NO: | 7365-45-9 |
| Purity: | >99.5% (Titration) |
| Chemical Formula: | C₁₀H₂₁NO₄S |
| Mol. Weight : | 238.30 g/mol |
| Useful pH Range: | 6.8-8.2 |
| pKa (25 °C): | 7.5 |
| Packages: | 500g/1Kg/25Kg/Custom Packaging |
Applications:
- It is a very popular buffering agent in cell culture media; and electroporation.
- Could be used as a running buffer in gel electrophoresis; studies related to the pH effects on seed germination. and can be used as a grinding buffer in plant studies.
- Also used as a binding buffer for protein studies and an eluent during cation exchange chromatography.